Facility WEHI

 WEHI provides:

  • CRISPR

The Melbourne Advanced Genome Editing Centre (MAGEC) was established in 2013 by the Herold lab, with the aim of developing CRISPR/Cas9 technology for gene editing within WEHI. The lab was able to adapt the CRISPR/Cas9 system into a novel inducible lentiviral platform for rapid and efficient modification of genes in cell lines and primary cells (Aubrey et al., Cell Reports 2015). During this time, MAGEC successfully translated their expertise in CRISPR/Cas9 technology into the generation of gene-targeted mice.

To date, the MAGEC lab has successfully used CRISPR/Cas9 to generate ~ 100 mouse models with constitutive gene knockouts, conditional alleles, epitope tags, single-base substitutions and gene knock-ins. The lab is highly experienced in designing gene targeting strategies, including sgRNA design, generating vectors for homologous recombination and genotyping targeted mice with next-generation sequencing.

The mouse production at WEHI is performed within an extremely clean animal facility, ready to distribute the mice within Australia without any quarantine requirements of the receiving institution. The MAGEC lab performs all standard gene-targeting projects on the C57BL/6 background. However, special requests for gene targeting on other backgrounds can be accommodated.

Successful generation of mice will be validated by NGS or LRPCR.

For more information please contact magec@wehi.edu.au

WEHi staff

Contact

Dr Marco Herold

E:  magec@wehi.edu.au